Carcinogenesis, Teratogenesis & Mutagenesis ›› 2002, Vol. 14 ›› Issue (4): 218-221.doi: 10.3969/j.issn.1004-616x.2002.04.004

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THE STUDIES 0F APOPTOSIS 0N MELANOMA A375 AND B16 CELLS INDUCED BY ARSENIC TRIOXIDE

CHEN Zhi-wen1, XIA jun1, HU Shou-fen1, MA Dong-zhu2, WANG li1   

  1. 1. Departments of Biochemistry and Molecular Biology. Bengbu Medical College, Bengbu 233003, China; 2. Departments of Biochemistry and Molecular Biology, Fu Dan University of Medical School, Shanghai 200032, China
  • Received:2002-05-10 Revised:2002-07-10 Online:2002-10-30 Published:2002-10-30

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Abstract: Purpose : To study the effect s of arsenic t rioxide (As2O3) on the apoptosis in melanoma A375 and B16 cells. Methods : Apoptosis and necrosis in A375 and B16 cells were analysed by flow cytomet ry ; The serum biochemical parameters in mice concerned with the functions of heart , liver and kidney were also measured after the mice were t reated with As2O3 . Results : As2O3 could induce apoptosis and necrosis of A375 and B16 cells. The rates of apoptosis in A375 and B16 cells were 11. 85 % , 55. 51 % , 54. 90 % , and 9. 81 % , 26. 45 % , 9. 93 % ,respectively , as t reated with different doses of As2O3 (5μmol·L - 1 , 10μmol·L - 1 and 20μmol·L - 1) ,respectively. The total rates of apoptosis and necrosis were 11. 90 % , 55. 71 % , 57. 40 % and 12. 96 % , 26. 99 % ,87. 13 %, respectively. No significant differences were found in serum biochemical parameters in mice t reated with As2O3 . Conclusion : As2O3 can induce apoptosis and necrosis of A375 and B16 cells , but does not cause damage to the functions of liver , kidney and heart in mice.

Key words: arsenic trioxide, melanoma, apoptosis, A375 cell, B16 cells

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